yflG [2019-06-17 12:06:19]

methionine aminopeptidase

Locus: BSU07690

Isoelectric point: 5.58

Molecular weight: 27.06 kDa

Protein length: 249 aa

Gene length: 750 bp

Function: removal of N-terminal methionine from nascent proteins

Product: methionine aminopeptidase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Translation/ other
• Information processing → Protein synthesis, modification and degradation → Protein modification → Protein maturation

Gene

Coordinates: 839,735 → 840,484

The protein

Catalyzed reaction/ biological activity

• Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides (according to Swiss-Prot)

Protein family

• peptidase M24A family (according to Swiss-Prot)

Paralogous protein(s)

• Map

Structure

• 1QXW (from Staphylococcus aureus, 51% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yflG-yflH-yflI
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigB: sigma factor, PubMed, in SigB regulon

  Regulatory mechanism

  • NagR: repression, (weak and not certain) PubMed, in NagR regulon

  Regulation

  • induced by stress (SigB) PubMed

Biological materials

Mutant

• MGNA-C255 (yflG::erm), available at the NBRP B. subtilis, Japan
• BKE07690 (ΔyflG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTGTCATTCCCGCTTTC, downstream forward: _UP4_TAAAACACATTCCGGGCTTC
• BKK07690 (ΔyflG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTGTCATTCCCGCTTTC, downstream forward: _UP4_TAAAACACATTCCGGGCTTC

References

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